INCREASED EXPRESSION LEVEL OF ADAPTOR PROTEIN Ruk/CIN85 IN DOXORUBICIN-RESISTANT HUMAN NON-SMALL LUNG ADENOCARCINOMA MOR CELLS IS ASSOCIATED WITH THEIR METABOLIC REPROGRAMMING

The aim of the present study was to find out role Ruk/CIN85 in modulation activities/content key enzymes/components glycolysis and hydrogen peroxide using as a model human NSCLC MOR wild type resistant drugs MOR/0.2R cells. Materials methods. (ECACC 84112312) 96042335), drug-resistant cell line, were cultured complete RPMI medium under standard conditions. Enzymes activity, content metabolites protein extracts conditioned culture estimated by spectrophotometric fluorometric assays. Results. Using RT2-PCR it revealed that level mRNA cells 10 times higher than parental activities lysyl oxidase (LOX) diamine (DAO) significantly It has been shown these enzymes are associated with aggressiveness tumor Based on obtained results, we draw conclusion observed changes intensity glycolysis, amine oxidases doxorubicin-resistant positively correlate expression adaptor studied. Conclusions. In conclusion, can be assumed is involved metabolome reprogramming may function an important component regulatory networks required for acquisition drug phenotype